Characterization of immobilized enzymes by flow-injection techniques with variable forward flow
| Autor: | Josef Flossdorf, Jaromir Ruzicka |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Chromatography
Immobilized enzyme biology technology industry and agriculture Forward flow equipment and supplies complex mixtures Biochemistry Horseradish peroxidase Analytical Chemistry chemistry.chemical_compound chemistry Flow (mathematics) Bioreactor biology.protein Environmental Chemistry Glucose oxidase Hydrogen peroxide Spectroscopy Peroxidase |
| Zdroj: | Analytica Chimica Acta. 218:291-301 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/s0003-2670(00)80305-0 |
| Popis: | The use of variable forward flow with small packed enzyme reactors is shown to be valuable for improving the efficiency of enzymatic conversion. Designs with stopped flow, oscillating flow and variable (fast/slow/fast) flow are compared for the spectrophotometric determination of glucose with glucose oxidase and horseradish peroxidase immobilized on controlled-pore glass in the same reactor. Variable forward flow increased the sensitivity considerably without excessive time consumption. The technique is also useful for characterizing the activity of immobilized enzyme reactors, e.g., peroxidase reactors for hydrogen peroxide determinations. |
| Databáze: | OpenAIRE |
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