1754-P: Follistatin-Like Protein 1 (FSTL1) Plays an Important Role in Human Primary Adipocyte Metabolism

Autor:	Chul Woo Ahn, Minkyung Kim, Yu Sik Kim, Shinae Kang, Ji Sun Nam, Sang Bae Lee, Jungeun Nam, Jung Hye Kim, Jong Suk Park, Kahui Park, Su Jeong Park, Arim Choi
Rok vydání:	2019
Předmět:	biology Akt/PKB signaling pathway Endocrinology Diabetes and Metabolism Adipose tissue AMPK Energy homeostasis Cell biology chemistry.chemical_compound chemistry Adipocyte Internal Medicine biology.protein Perilipin Lipolysis Follistatin
Zdroj:	Diabetes. 68
ISSN:	1939-327X 0012-1797
Popis:	Objective: FSTL1, an extracellular glycoprotein, is a novel myokine that is secreted by skeletal muscle. However, its functions in metabolism remain unclear, Adipose tissue is one of the important organs for maintaining energy homeostasis. FSTL1 is known to induce inflammatory response and inhibit insulin-mediated Akt signaling pathway, we aimed to investigate whether FSTL1 play metabolic roles in human adipose tissue. We hypothesized that FSTL1 increases lipolysis through its putative receptor DIP2A (Disco interacting protein 2 homolog A). Methods: Human primary adipocytes were treated with recombinant FSTL1 in dose and time dependent manner. To determine its lipolytic activity, pHSL ser563,565,660, perilipin, AMPK, PKA were determined by western blot and the rate of free fatty acid was assessed. Expression of DIP2A was determined by PCR and western blot. DIP2A was knocked-down to examine whether it mediates metabolic effects of FSTL1. Results: FSTL1 did not affect catecholamine-induced lipolysis via PKA. FSTL1, however, increased lipolysis through AMPK activation and, subsequently, HSL ser565 phosphorylation in dose dependent manner. It was found that DIP2A expression did not change during differentiation of human primary adipocyte, suggesting that it is not involved in adipocyte maturation. Conclusion: FSTL1 increased basal lipolysis through AMPK activation. AMPK is well known sensor of the intracellular energy state and serves to regulate various signals. Its activation provides ATP by FA oxidation. Therefore, FSTL1 may play an important in the regulation of energy homeostasis. FSTL1 also has effect through DIP2A. These findings suggest that DIP2A plays important roles in FSTL1 mediated lipolysis. Disclosure C. Ahn: None. A. Choi: None. J. Kim: None. K. Park: None. S. Lee: None. J. Nam: None. S. Park: None. M. Kim: None. Y. Kim: None. J. Nam: None. S. Kang: None. J. Park: None.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f3313e260c306627f4d8cc24c6f7acff https://doi.org/10.2337/db19-1754-p Zobrazit plný text záznamu