Characterization of experimental spinal cord injury with magnetization transfer ratio histograms
| Autor: | Jeffery I. Berman, J. Chetley Ford, Joseph C. McGowan, David B. Hackney, Ehud Lavi |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.diagnostic_test
Chemistry medicine.medical_treatment Laminectomy Magnetic resonance imaging Anatomy medicine.disease Spinal cord White matter medicine.anatomical_structure Injury Site Edema medicine Radiology Nuclear Medicine and imaging Magnetization transfer medicine.symptom Spinal cord injury |
| Zdroj: | Journal of Magnetic Resonance Imaging. 12:247-254 |
| ISSN: | 1522-2586 1053-1807 |
| DOI: | 10.1002/1522-2586(200008)12:2<247::aid-jmri6>3.0.co;2-x |
| Popis: | This study was designed to characterize the severity of tissue damage in experimental spinal cord injury using magnetization transfer (MT) histogram analysis. Seven Sprague-Dawley rats were subjected to laminectomy and standard weight-drop injury to the spinal cord (four rats at 15 cm drop-height and three rats at 2.5 cm). Three control animals underwent laminectomy without weight-drop. After sacrifice, the animals were scanned at 1.9 T with a pulsed off-resonance MT technique. Following magnetic resonance (MR) imaging, the cords were embedded in paraffin and sectioned into 5-microm sections for semiquantitative histopathological analysis. Composite histograms were generated using data spanning an axial distance of 3 cm centered on the injury site. MT histogram parameters, such as the amount of tissue with statistical correspondence to normal white matter, were highly predictive of histopathological results, including myelination state and neurofilament damage. Less correlation with edema was observed, suggesting that the technique was most sensitive to true tissue alteration. |
| Databáze: | OpenAIRE |
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