Increased apoptosis of peripheral blood T cells following allogeneic hematopoietic cell transplantation

Autor:	Alexandre Barsoukov, Ming Tseh Lin, Ted Gooley, Haydar Frangoul, Li Hui Tseng, John A. Hansen, Yoshiki Akatsuka, Ji Pei
Rok vydání:	2000
Předmět:	medicine.medical_treatment Immunology Cell Biology Hematology Hematopoietic stem cell transplantation Biology Biochemistry Andrology Transplantation Immune system Antigen Apoptosis Lymphocyte homeostasis medicine Lymphopoiesis CD8
Zdroj:	Blood. 95:3832-3839
ISSN:	1528-0020 0006-4971
Popis:	Lymphopenia and immune deficiency are significant problems following allogeneic hematopoietic cell transplantation (HCT). It is largely assumed that delayed immune reconstruction is due to a profound decrease in thymus-dependent lymphopoiesis, especially in older patients, but apoptosis is also known to play a significant role in lymphocyte homeostasis. Peripheral T cells from patients who received HCT were studied for evidence of increased cell death. Spontaneous apoptosis was measured in CD3+ T cells following a 24-hour incubation using 7-amino-actinomycin D in conjunction with the dual staining of cell surface antigens. Apoptosis was significantly greater among CD3+ T cells taken from patients 19-23 days after transplantation (30.4% ± 12.5%,P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::f1dcddbfda690623231becf723965a63 https://doi.org/10.1182/blood.v95.12.3832 Zobrazit plný text záznamu