Sequence Mapping of Epoxide Adducts in Human Hemoglobin with LC−Tandem MS and the Salsa Algorithm
| Autor: | Daniel C. Liebler, Hamid Badghisi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Chemical Research in Toxicology. 15:799-805 |
| ISSN: | 1520-5010 0893-228X |
| Popis: | The rapid development and integration of liquid chromatography-tandem mass spectrometry (LC-MS-MS) has enabled the high-throughput identification of proteins and driven the expanding field of proteomics. LC-MS-MS also offers an attractive general approach to the analysis of xenobiotic adducts on proteins. The aim of this study was to examine the combined use of LC-MS-MS and the SALSA algorithm as a general approach to map xenobiotic adducts on proteins at the level of amino acid sequence. Hemoglobin (Hb) adducts are commonly used as biomarkers for exposure to environmental toxicants. Human Hb was incubated with styrene oxide, ethylene oxide, and butadiene dioxide (40 mM) to form adducts, digested with trypsin and analyzed by LC-MS-MS on a ThermoFinnigan LCQ ion trap MS instrument. Data-dependent scanning was used for acquisition of MS-MS spectra. The SALSA algorithm was used to detect MS-MS spectra of native and modified Hb peptides. The adducted sites identified are the N-terminal valines of both Hbα and... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|