DNA polymerase η is an A-T mutator in somatic hypermutation of immunoglobulin variable genes

Autor:	Kenneth H. Kraemer, Alan R. Lehmann, Xianmin Zeng, Patricia J. Gearhart, David B. Winter, Cynthia Kasmer
Rok vydání:	2001
Předmět:	Genetics Mutation Xeroderma pigmentosum biology DNA polymerase Base pair Immunology Somatic hypermutation medicine.disease medicine.disease_cause Molecular biology biology.protein medicine Immunology and Allergy Antibody Gene Polymerase
Zdroj:	Nature Immunology. 2:537-541
ISSN:	1529-2916 1529-2908
Popis:	To determine whether DNA polymerase plays a role in the hypermutation of immunoglobulin variable genes, we examined the frequency and pattern of substitutions in variable VH6 genes from the peripheral blood lymphocytes of three patients with xeroderma pigmentosum variant disease, whose polymerase had genetic defects. The frequency of mutation was normal but the types of base changes were different: there was a decrease in mutations at A and T and a concomitant rise in mutations at G and C. We propose that more than one polymerase contributes to hypermutation and that if one is absent, others compensate. The data indicate that polymerase is involved in generating errors that occur predominantly at A and T and that another polymerase(s) may preferentially generate errors opposite G and C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed04f49b177c88fa28773009e865ca79 https://doi.org/10.1038/88740 Zobrazit plný text záznamu Plný text ve formátu PDF