Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery
Autor: | Anas El-Aneed, Mays Al-Dulaymi, Waleed Mohammed-Saeid, Ildiko Badea |
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Rok vydání: | 2019 |
Předmět: |
chemistry.chemical_classification
0303 health sciences Low toxicity Biocompatibility Chemistry Cationic polymerization Peptide 02 engineering and technology Transfection Gene delivery 021001 nanoscience & nanotechnology In vitro transfection Combinatorial chemistry 03 medical and health sciences Pulmonary surfactant lipids (amino acids peptides and proteins) 0210 nano-technology 030304 developmental biology |
Zdroj: | Pharmaceutical Nanotechnology ISBN: 9781493995158 |
Popis: | Diquaternary ammonium-based gemini surfactants have been investigated widely as nonviral gene delivery systems. These unique cationic lipids have versatility in their chemical structure, show relatively low toxicity, are able to compact genetic material (pDNA, RNA) into nano-sized lipoplexes, and can be easily produced. In addition, the gemini surfactants show significant improvement in the transfection activity and biocompatibility compared to other cationic lipids used as nonviral gene delivery agents. The successful applications of gemini surfactant-based lipoplexes as topical gene delivery systems in animal models indicate their potential as noninvasive carriers for genetic immunization, theranostic agents, and in other gene therapy treatments. Detailed physicochemical characterization of gemini surfactant lipoplexes is a key factor in terms of formulation optimization and elucidation of the cellular uptake and stability of the lipoplexes system. In this chapter, we describe in detail different formulation methods to prepare gemini surfactant lipoplexes and comprehensive physicochemical characterization. In addition, we illustrate general protocols for in vitro evaluations. |
Databáze: | OpenAIRE |
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