In vivo effects of zoledronic acid on oral mucosal epithelial cells
| Autor: | T-M Chu, L. Jack Windsor, Ahmed Ghoneima, Allen, Eman Allam |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology TUNEL assay biology business.industry Caspase 3 Matrix metalloproteinase Proliferating cell nuclear antigen Endocrinology Zoledronic acid medicine.anatomical_structure Otorhinolaryngology Apoptosis In vivo Internal medicine medicine biology.protein Oral mucosa business General Dentistry medicine.drug |
| Zdroj: | Oral Diseases. 17:291-297 |
| ISSN: | 1354-523X |
| DOI: | 10.1111/j.1601-0825.2010.01739.x |
| Popis: | Objective—Osteonecrosis of the jaw is a serious complication of bisphosphonate treatment for which the pathophysiology is unknown. The purpose of this study was to investigate whether in vivo zoledronic acid (ZA) induces alterations in cell proliferation, apoptosis, and matrix metalloproteinases (MMPs) expression in oral mucosal epithelial cells. Methods—One year old dogs were either untreated (control group) or given high doses of intravenous ZA (ZA group) for three months. The doses of ZA were equivalent to those given to cancer patients, yet were administered two times more frequently (every 2 weeks). Mucosal tissues were assessed immunohistochemically for cell proliferation (proliferating cell nuclear antigen, PCNA), matrix metalloproteinase (MMP) expression, and apoptosis (caspase 3 and TUNEL). Results—There were no significant differences between the groups with respect to PCNA, MMP-2, MMP-14, and TUNEL positive cells. However, the expression of MMP-9 was significantly higher in the control group than in the ZA group (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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