Evaluation of a Diagnostic Method to Quantify Aflatoxins B1 and M1 in Animal Liver by High-Performance Liquid Chromatography with Fluorescence Detection
| Autor: | Andriy Tkachenko, Lori Smith, Kyle A. Francis, Paula M. Imerman, Xiangwei Du, John M. Tahara, Renate Reimschuessel, Wilson K. Rumbeiha, Dwayne E. Schrunk |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
Aflatoxin Reproducibility Chromatography Diagnostic methods Intralaboratory 010405 organic chemistry Chemistry 010401 analytical chemistry Repeatability 01 natural sciences High-performance liquid chromatography Method test 0104 chemical sciences Analytical Chemistry surgical procedures operative Environmental Chemistry Agronomy and Crop Science Quantitation Range Food Science |
| Zdroj: | Journal of AOAC International. 102:1530-1534 |
| ISSN: | 1060-3271 |
| DOI: | 10.5740/jaoacint.18-0355 |
| Popis: | Background: Aflatoxins (AFs) are secondary metabolites of fungi and are one of the causes of toxin-related pet food recalls. An intralaboratory method was previously developed to quantify aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) in animal liver by HPLC with fluorescence detection. Objective: The aim of this study was to extensively evaluate the method performance with a single-laboratory blinded method test (BMT-S) and a multilaboratory blinded method test (BMT-M). Methods: Blinded tissue samples were prepared by a third-party laboratory and sent out to participating laboratories for both BMT-S and BMT-M. Results: In both tests, participants analyzed blinded samples prepared by an independent laboratory. In the BMT-S, accuracy ranged between 111 and 154% for AFB1 and 113 and 159% for AFM1 within the quantitation range of 0.1-0.5 ng/g. The HorRat values for repeatability ranged between 0.1 and 0.3 for AFB1 and 0.3 and 0.6 for AFM1. In the BMT-M, the interlaboratory accuracy ranged between 77 and 81% for AFB1 and 83 and 85% for AFM1 within the quantitation range of 0.2-10 ng/g. The HorRat values for reproducibility ranged between 0.4 and 0.7 for AFB1 and 0.4 and 0.9 for AFM1. Both recovery and reproducibility were acceptable. Conclusions: BMT-M evaluation demonstrated that the method was suitable for quantitation of aflatoxins B1 and M1 in animal liver between laboratories. Highlights: The BMT-S and BMT-M results demonstrated that the method is rugged and reproducible among the participating laboratories. |
| Databáze: | OpenAIRE |
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