Validation of a Multiplex PCR Assay for the Forensic Identification of Indian Crocodiles*
| Autor: | Kothakota Naga Jogayya, Bhawna Dubey, Ikramul Haque, P. R. Meganathan |
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| Rok vydání: | 2011 |
| Předmět: |
Validation study
biology business.industry biology.organism_classification Crocodylus Pathology and Forensic Medicine Biotechnology Forensic identification DNA profiling Multiplex polymerase chain reaction Genetics Forensic engineering Species identification Identification (biology) business Analysis method |
| Zdroj: | Journal of Forensic Sciences. 56:1241-1244 |
| ISSN: | 0022-1198 |
| DOI: | 10.1111/j.1556-4029.2011.01812.x |
| Popis: | A dependable and efficient wildlife species identification system is essential for swift dispensation of the justice linking wildlife crimes. Development of molecular techniques is befitting the need of the time. The forensic laboratories often receive highly ill-treated samples for identification purposes, and thus, validation of any novel methodology is necessary for forensic usage. We validate a novel multiplex polymerase chain reaction assay, developed at this laboratory for the forensic identification of three Indian crocodiles, Crocodylus palustris, Crocodylus porosus, and Gavialis gangeticus, following the guidelines of Scientific Working Group on DNA Analysis Methods. The multiplex PCR was tested for its specificity, reproducibility, sensitivity, and stability. This study also includes the samples treated with various chemical substances and exposed to various environmental regimes. The result of this validation study promises this technique to be an efficient identification tool for Indian crocodiles and therefore is recommended for forensic purposes. |
| Databáze: | OpenAIRE |
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