Robo1-specific CAR-NK Immunotherapy Enhances Efficacy of 125I Seed Brachytherapy in an Orthotopic Mouse Model of Human Pancreatic Carcinoma
| Autor: | Robert M. Hoffman, Zhijian Yang, Zhongmin Wang, Ju Gong, Pang Haopeng, Fen-Ju Liu, Ning Xia, Jian Lu, Y U Sun, Zhijin Chen, Yunfeng Zheng |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
medicine.diagnostic_test business.industry medicine.medical_treatment Cell Brachytherapy Magnetic resonance imaging General Medicine Immunotherapy medicine.disease 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Oncology Antigen Pancreatic tumor ROBO1 030220 oncology & carcinogenesis Pancreatic cancer medicine Cancer research business |
| Zdroj: | Anticancer Research. 39:5919-5925 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13796 |
| Popis: | BACKGROUND/AIM The aim of the current study was to investigate the synergistic efficacy of Robo1 bichimeric antigen receptor-natural killer cell (BiCAR-NK) immunotherapy and 125I seed brachytherapy in an orthotopic pancreatic cancer mouse model. MATERIALS AND METHODS The orthotopic pancreatic tumor model was established with human pancreatic cancer BxPC-3 cells expressing red fluorescent protein. The mice were treated with 125I seed implantation alone or the combination of 125I seeds with Robo1-specific CAR-NK cells. To assess tumor inhibition, in vivo fluorescence imaging was conducted. 7 Tesla magnetic resonance (7T-MR) scanning was applied to measure the changes in the metabolic profiles of tumor tissues. RESULTS Tumor size was significantly reduced in the 125I and 125I +CAR-NK treated group compared to the untreated group (p |
| Databáze: | OpenAIRE |
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