Assessing membrane charging by alternating electric fields in giant unilamellar vesicles

Autor: Esin B. Sozer, Mara Casebeer, Bennett L. Ibey, Zachary Coker, Stacey L. Martens, Allen S. Kiester, Joel N. Bixler
Rok vydání: 2021
Předmět:
Zdroj: Optical Interactions with Tissue and Cells XXXII.
Popis: Our group recently published direct observation of membrane charging in FluoVolt labeled CHO-K1 cells by nanosecond electrical pulses using a streak camera. Using this technique, called Streak Camera Microscopy (SCM), we imaged the membrane charging dynamics in giant unilamellar vesicles (GUVs) during AC exposures up to 6 MHz and compared these results to existing capacitive circuit models of membranes. This work shows further application of Streak Camera Microscopy for evaluation of high speed biological events.
Databáze: OpenAIRE