| Popis: |
The body muscle is an important tissue used in organisms for proper viability and locomotion. Although this tissue is generally well studied and characterized, and many pathways have been elucidated throughout the years, we still lack a comprehensive understanding of its transcriptome and how it controls muscle development and function. Here, we have updated a nuclear FACS sorting-based methodology to isolate and sequence a high-quality muscle transcriptome fromC. elegansmixed-stage animals. We have identified 2,848 muscle-specific protein-coding genes, including 78 transcription factors and 206 protein-coding genes containing an RNA binding domain. We studied their interaction network, performed a detailed promoter analysis, and identified novel muscle-specificcis-acting elements. We have also identified 16 high-quality muscle-specific miRNAs, studied their functionin vivousing fluorochrome-based analyses, and developed a high-qualityC. elegansmiRNA Interactome incorporating other muscle-specific datasets produced by our lab and others.Our study expands our understanding of how muscle tissue functions inC. elegansand in turn, provide results that can in the future be applied to humans to study muscular-related diseases. |
