Plasma pharmacokinetics and tissue distribution of L-lysine α-oxidase from Trichoderma cf. aureoviride RIFAI VKM F- 4268D in mice
| Autor: | S Sh Karshieva, E. V. Lukasheva, A. Yu. Arinbasarova, Vadim S. Pokrovsky, M V Komarova, A. G. Medentzev, Gulalek Babayeva, Alexander N. Lukashev |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Oxidase test 030102 biochemistry & molecular biology medicine.diagnostic_test Organic Chemistry Clinical Biochemistry Lysine Spleen Antimicrobial L-amino-acid oxidase Biochemistry Molecular biology 03 medical and health sciences 030104 developmental biology Enzyme medicine.anatomical_structure chemistry Pharmacokinetics Immunoassay medicine |
| Zdroj: | Amino Acids. 53:111-118 |
| ISSN: | 1438-2199 0939-4451 |
| DOI: | 10.1007/s00726-020-02930-4 |
| Popis: | L-lysine α-oxidase (LO) is an L-amino acid oxidase with antitumor, antimicrobial and antiviral properties. Pharmacokinetic (PK) studies were carried out by measuring LO concentration in plasma and tissue samples by enzyme immunoassay. L-lysine concentration in samples was measured spectrophotometrically using LO. After single i.v. injection of 1.0, 1.5, 3.0 mg/kg the circulating T1/2 of enzyme in mice varied from 51 to 74 min and the AUC0–inf values were 6.54 ± 0.46, 8.66 ± 0.59, 9.47 ± 1.45 μg/ml × h, respectively. LO was distributed in tissues and determined within 48 h after administration with maximal accumulation in liver and heart tissues. Mean time to reach the maximum concentration was highest for the liver—9 h, kidney—1 h and 15 min for the tissues of heart, spleen and brain. T1/2 of LO in tissues ranged from 7.75 ± 0.73 to 26.10 ± 2.60 h. In mice, plasma L-lysine decreased by 79% 15 min after LO administration in dose 1.6 mg/kg. The serum L-lysine levels remained very low from 1 to 9 h ( |
| Databáze: | OpenAIRE |
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