Spatial regulation of microtubule disruption during dendrite pruning in Drosophila
| Autor: | Sebastian Rumpf, Lea-Franziska Reekers, Ina Götzelmann, Svende Herzmann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Neurite fungi Dendrite Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Tubulin medicine.anatomical_structure nervous system Microtubule Live cell imaging biology.protein medicine Kinesin Soma Molecular Biology 030217 neurology & neurosurgery Pruning (morphology) Developmental Biology |
| Zdroj: | Development. |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.156950 |
| Popis: | Large scale neurite pruning is an important specificity mechanism during neuronal morphogenesis. Drosophila sensory neurons prune their larval dendrites during metamorphosis. Pruning dendrites are severed in their proximal regions, but how this spatial information is encoded is not clear. Dendrite severing is preceded by local breakdown of dendritic microtubules through PAR-1-mediated inhibition of Tau. Here, we investigated spatial aspects of microtubule breakdown during dendrite pruning. Live imaging of fluorescently tagged tubulin shows that microtubule breakdown first occurs at proximal dendritic branchpoints, followed by breakdown at more distal branchpoints, suggesting that the process is triggered by a signal emanating from the soma. In fly dendrites, microtubules are arranged in uniformly oriented arrays where all plus ends face towards the soma. Mutants in kinesin-1 and -2, which are required for uniform microtubule orientation, cause defects in microtubule breakdown and dendrite pruning. Our data suggest that the local microtubule organization at branch points determines where microtubule breakdown occurs. Local microtubule organization may therefore contribute spatial information for severing sites during dendrite pruning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|