| Popis: |
Some microorganisms identified in cave ecosystems have been reported to play a permanent and significant role for maintaininglife in such environments. Human entrance into caves can induce some changes on cave physic-ochemical parameters which altimately affects the living organisms. In this regard, for the first time, Morca Cave was explored to evaluate the impacts that human activities may have on the microbial diversity of the cave in a limited period of time. During this expedition at a depth of 1040 m, a camp was established for four days. Before the installation and at the end of the camp, sediments and surface samples were taken from different points of the camp area and the area around it. Sequencing of 16s rRNA of each sample was performed using the next generation sequencing method. The profile of the microbial diversity before the camping reaveled that Thermoplasmata dominated the archaea group and Gamma- and Alpha-proteobacteria were the most dominant bacterial group. After the camp, a decrease in the microbial diversity especially the previously mentioned classes strains is observed at the most of the sampled areas. The results also showed that Bacilli strains significantly increased after the camp and increase of Bacteroidia strains is observed at the most active sampled areas. This present study therefore highlights how microbial diversity inside a closed cave can respond to the human activities within a short period. Furthermore, it may constitute a solid basis to support efforts targeted at improving technics for cave management and expedition for the conservation of cave nature. |
