Comparison between Ca2+-induced scrambling of various fluorescently labelled lipid analogues in red blood cells

Autor:	David W. C. DEKKERS, Paul COMFURIUS, Edouard M. BEVERS, Robert F. A. ZWAAL
Rok vydání:	2002
Předmět:	technology industry and agriculture lipids (amino acids peptides and proteins) Cell Biology Molecular Biology Biochemistry
Zdroj:	Biochemical Journal. 362:741-747
ISSN:	1470-8728 0264-6021
DOI:	10.1042/bj3620741
Popis:	Treatment of red blood cells with calcium and ionomycin causes activation of the lipid scramblase, a putative membrane protein catalysing flip-flop of (phospho)lipids. Various fluorescent 1-oleoyl-2-[6(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino] caproyl (C6-NBD) analogues were tested for transbilayer movement across the plasma membrane of red blood cells. Among these phospholipid analogues were phosphatidylgalactose, phosphatidylmaltose and phosphatidylmaltotriose, which were obtained from C6-NBD-phosphatidylcholine by phospholipase D-catalysed transphosphatidylation. The inward movement after the onset of scrambling was monitored by extraction of the non-internalized probe with BSA. We demonstrate that both the amino group and the size of the headgroup determine the kinetics of lipid scrambling, and that lipids with a ceramide backbone migrate much more slowly than glycerophospholipids with the same headgroup.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e9e8a17a32e0cf18ea1823b55d642cc4 https://doi.org/10.1042/bj3620741 Zobrazit plný text záznamu