Autor: |
Flávio Pinto Monção, Luana Sabrine Silva, Denilson F. Oliveira, Edson Hiydu Mizobutsi, Marcos Paz Saraiva Câmara, Regina Cássia Ferreira Ribeiro, Viviane Aparecida Costa Campos, Gisele Polete Mizobutsi, Maria Luisa Mendes Rodrigues |
Rok vydání: |
2019 |
Předmět: |
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Zdroj: |
Journal of Experimental Agriculture International. :1-8 |
ISSN: |
2457-0591 |
Popis: |
Aims: To evaluate the in vitro effect of mango peel extracts using different types of solvent and concentrations on the Colletotrichum gloeosporioides development. Study Design: Activities were aimed at evaluating the in vitro antifungal potential of mango peel extracts. Study Location and duration: The study was carried out at the Laboratory of Post-Harvest Pathology of Fruits and Vegetables - State University of Montes Claros and Laboratory of Natural Products, Department of Chemistry - Federal University of Lavras during October and December 2018. Methodology: ‘Palmer’ mango peel (Mangifera indica) was submitted to drying in oven and grinding. Subsequently, extracts were obtained in Soxhlet system, using methanol, ethyl acetate and hexane as solvents. The three extracts were tested in vitro at concentrations of 0.0; 0.25; 0.5; 1.0 and 2.0 mg/mL by adding them in culture medium against C. gloeosporioides, which was isolated from mango fruits with anthracnose symptoms. The effect of extracts and their respective concentrations on the mycelial growth rate and conidia production and germination was evaluated. The design was completely randomized in a 3 x 5 factorial arrangement with 5 replicates. Results: Increased extract concentrations caused reduction in the mycelial growth rate of the pathogen (R2 = 0.96). Both factors under study acted simultaneously in conidia production (P < 0.05), and the hexane extract presented better results for this analyzed variable. There was total germination inhibition (P < 0.05) when 1 mg/mL ethyl acetate extract and 2 mg/mL methanol and hexane extracts were used. Conclusion: Methanol, hexane and ethyl acetate mango peel extracts had inhibitory effect on the in vitro C. gloeosporioides development. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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