Detection of peroxynitrite anion released from HeLa cells via hyper-cross-linked polymer nanotube modified electrode
| Autor: | Xiuhui Liu, Weizhou Fan, Caixiu Luo, Fuxin Liu, Qian Liu, Fangping Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Nanotube Chemistry Scanning electron microscope General Chemical Engineering General Engineering General Physics and Astronomy 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry Electrochemistry 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Transmission electron microscopy Electrode General Materials Science Cyclic voltammetry 0210 nano-technology Peroxynitrite |
| Zdroj: | Ionics. 27:1331-1337 |
| ISSN: | 1862-0760 0947-7047 |
| DOI: | 10.1007/s11581-020-03887-w |
| Popis: | Abnormal expression levels of peroxynitrite anion (ONOO−) in human cells have relation to many diseases, especially cancer. Here, we constructed a novel sensing platform for directly detecting ONOO− using hyper-cross-linked polymeric nanotubes (HNTs)–modified glassy carbon electrode. The HNTs nanohybrids were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), FT-IR spectroscopy, and cyclic voltammetry (CV). The prepared HNTs/GCE sensor exhibited outstanding performance in detecting ONOO−, which provided a wide linear range from 2.34 × 10−8 M to 1.74 × 10−4 M and a low detection limit of 7.43 nM (S/N = 3). Moreover, it was also used to measure the ONOO− released from HeLa cells induced by stimulus. The designed sensor may provide great potential for expand applications in the matter of cancer treatment and biomedicine. |
| Databáze: | OpenAIRE |
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