Abstract P114: Chemerin Leads to a Contraction of the Uterine Artery After Stimulation With Seretonin
| Autor: | Victor V. Lima, Vanessa Dela Justina, Fernanda R. Giachini, Clinton Webb, Fernanda Priviero |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Hypertension. 74 |
| ISSN: | 1524-4563 0194-911X |
| Popis: | Chemerin circulating levels are augmented during preeclampsia. This adipokine has been shown to contract many vascular beds, however, its role on the uterine artery (UA) remains to be elucidated. This study tests the hypothesis that chemerin leads to a hypercontractile state of the uterine artery contributing to the development of preeclampsia-like symptoms. Also chemerin may interacts with serotonin, which is released by the placenta and increased in preeclampsia, to impair the circulation of uterine artery during preeclampsia. UA from pregnant Sprague Dawley rats (200-250g) were removed and mounted in wire myograph with or without PVAT. Concentration response-curves to thromboxane analogue [U-46619 (1 nM to 3μM)] and seretonin [5-HT (1 nM to 100 μM)] were performed in UA and repetead in the presence of chemerin (0.5 ng/ml and 5 ng/ml, 1 hour). Incubation with chemerin 5ng/ml led to contraction of the UA when administered after concentration-response curve to 5-HT (68% contraction; 17 of 25mN), but not U-46619. Neither the potency nor the maximal response of 5-HT and U-46619 were changed in the presence of chemerin. Contraction induced by chemerin (5ng/ml) was not seen in UA with PVAT. Chemerin 0.5ng/ml did not contract the UA and did not cause changes in agonist-induced contractions. Chemerin 5ng/ml leads to a contraction of the UA after stimulation with 5-HT which was prevented in the presence of PVAT. We conclude that signaling pathway stimulated by 5-HT unvel the contractile properties of the adipokine. These tow vasoactive agents may come together to elevate blood pressure in preeclampsia. |
| Databáze: | OpenAIRE |
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