Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG
| Autor: | Maryam Mousivand, Seyyed Mohammad Mousavi, Hadis Moteshafi, Maryam Hashemi |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Central composite design General Chemical Engineering Bacillus subtilis biology.organism_classification 01 natural sciences Xylan Reducing sugar 03 medical and health sciences 030104 developmental biology chemistry 010608 biotechnology Xylanase Food science Response surface methodology Digestion Phyllosphere |
| Zdroj: | Journal of Industrial and Engineering Chemistry. 37:251-260 |
| ISSN: | 1226-086X |
| DOI: | 10.1016/j.jiec.2016.03.036 |
| Popis: | Bacillus subtilis D3d newly isolated from apricot phyllosphere has the potential of producing high levels of xylanase. The present study used response surface methodology (RSM) to optimize catalytic activity. Factors including temperature, pH and beechwood xylan concentration were optimized using central composite design (CCD). Under optimized condition maximum xylanase activity found 260 U/ml. The enzyme showed stability over a range of pH (5.0–9.0) and exhibited more than 80% activity after 72 h at 40 °C. Such RSM models as well as stability data can be used at different operational conditions to predict the residual catalytic activity. Regard to pre-digested brewer's spent grain (BSG) has potential as a valuable poultry B. subtilis D3d crude xylanase. Maximum reducing sugar yield were found to be 68.43 mgreducing sugars/gBSG at optimum condition. The results revealed that B.subtilis D3d is a promising xylanase-producing bacterium with great potential for use in the food and feed industries. |
| Databáze: | OpenAIRE |
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