| Autor: |
Junfeng Hao, Shi-Fang Wu, Chun-Ling Zhu, Changgai Mu, Longchao Zhang, David M. Irwin, Lixian Wang, Jilong Ren, Ya-Ping Zhang, Yun Gao, Bingyu Mao, Hai-Bing Xie, Tang Hai, Ligang Wang, Dawei Yu, Adeniyi C. Adeola, Jianbo Li, Yongqing Zhang |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Thoracolumbar vertebra (TLV) and rib primordium (RP) development is a common evolutionary feature across vertebrates although whole-organism analysis of TLV and RP gene expression dynamics has been lacking. Here we investigated the single-cell transcriptomic landscape of thoracic vertebra (TV), lumbar vertebra (LV), and RP cells from a pig embryo at 27 days post-fertilization (dpf) and identified six cell types with distinct gene-expression signatures. In-depth dissection of the gene-expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and rib development. Further analysis of cell-type-specific and strand-specific expression uncovered the extremely high levels of HOXA10 3’-UTR sequence specific to osteoblast of LV cells, which may function as anti-HOXA10-antisense by counteracting the HOXA10-antisense effect to determine TLV transition. Thus, this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell-type-specific resolution, which serves as a comprehensive view on the transcriptional profile of animal embryo development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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