Protective effects of ellagic acid and ozone on rat ovaries with an ischemia/reperfusion injury
| Autor: | Arda Isik, Cebrail Gürsul, Kemal Peker, Ilyas Sayar, Senol Bicer, Mehmet Gürbüzel |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Glutathione reductase Ovary medicine.disease_cause Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Edema medicine 030219 obstetrics & reproductive medicine biology business.industry Obstetrics and Gynecology medicine.disease Malondialdehyde medicine.anatomical_structure Endocrinology chemistry 030220 oncology & carcinogenesis Anesthesia biology.protein medicine.symptom business Reperfusion injury Oxidative stress |
| Zdroj: | Journal of Obstetrics and Gynaecology Research. 42:52-58 |
| ISSN: | 1341-8076 |
| Popis: | Aim This study investigated the effects of the antioxidant agents, ozone (O) and ellagic acid (EA), on ischemia/reperfusion (I/R) injuries developed from an ovarian torsion-detorsion model. Materials and Methods Arteries in the left ovaries of rats were clamped for two hours to achieve torsion, and then the clamps were removed for a two-hour detorsion period. Thirty-five female Sprague–Dawley rats were randomly divided into five groups: control: administered only with anesthesia, rats were not subjected to torsion-detorsion; I/R: subjected to torsion and subsequent detorsion, without administering any treatment agent; and I/R + EA, I/R + O and I/R + O + EA: subjected to torsion and detorsion processes and administered with EA, O or EA + O at the 75th minute of torsion. The rats were then sacrificed under general anesthesia and the ovarian tissues were excised. The tissues were homogenized and levels of glutathione reductase, catalase, superoxide dismutase and malondialdehyde (MDA) were analyzed. Tissue damage was evaluated in terms of histopathological parameters, such as hemorrhage, congestion, edema and inflammation. Results Antioxidant enzyme activity and MDA levels in the ovary tissue increased in the I/R group and decreased in the O, EA and O + EA groups (P < 0.05). Histopathological examination revealed that tissue damage in the O, EA and O + EA groups decreased in comparison with the I/R group (P < 0.05). Conclusions These biochemical and histopathological findings suggest that EA and O are effective against ovarian I/R injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text