A Gain-of-Function Screen Reveals Redundant ERF Transcription Factors Providing Opportunities for Resistance Breeding Toward the Vascular Fungal Pathogen Verticillium longisporum
| Autor: | Elisabeth Walper, Christoph Weiste, Tim Iven, Wolfgang Dröge-Laser, Christian Fröschel, Vanessa Bachmann |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Regulation of gene expression biology Physiology fungi food and beverages General Medicine biology.organism_classification Verticillium 01 natural sciences Transcriptome 03 medical and health sciences Open reading frame 030104 developmental biology Verticillium longisporum Arabidopsis thaliana Agronomy and Crop Science Transcription factor Gene 010606 plant biology & botany |
| Zdroj: | Molecular Plant-Microbe Interactions®. 32:1095-1109 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-02-19-0055-r |
| Popis: | Verticillium longisporum is a vascular fungal pathogen leading to severe crop loss, particular in oilseed rape. Transcription factors (TF) are highly suited for genetic engineering of pathogen-resistant crops, as they control sets of functionally associated genes. Applying the AtTORF-Ex (Arabidopsis thaliana transcription factor open reading frame expression) collection, a simple and robust screen of TF-overexpressing plants was established displaying reduced fungal colonization. Distinct members of the large ethylene response factor (ERF) family, namely ERF96 and the six highly related subgroup IXb members ERF102 to ERF107, were identified. Whereas overexpression of these ERF significantly reduces fungal propagation, single loss-of-function approaches did not reveal altered susceptibility. Hence, this gain-of-function approach is particularly suited to identify redundant family members. Expression analyses disclosed distinct ERF gene activation patterns in roots and leaves, suggesting functional differences. Transcriptome studies performed on chemically induced ERF106 expression revealed an enrichment of genes involved in the biosynthesis of antimicrobial indole glucosinolates (IG), such as CYP81F2 (CYTOCHROME P450-MONOOXYGENASE 81F2), which is directly regulated by IXb-ERF via two GCC-like cis-elements. The impact of IG in restricting fungal propagation was further supported as the cyp81f2 mutant displayed significantly enhanced susceptibility. Taken together, this proof-of-concept approach provides a novel strategy to identify candidate TF that are valuable genetic resources for engineering or breeding pathogen-resistant crop plants. |
| Databáze: | OpenAIRE |
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