| Autor: |
Yuehan Wan, Jianwen Chen, Maosheng Cheng, Chen Demeng, Xiuyun Xu, Shuang Chen, Gan Xiong, Liang Peng, Ganping Wang, Cheng Wang, Zhi Chen, Xiaochen Wang, Kang Li |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-101161/v1 |
| Popis: |
Background: Accumulating evidence supports that Methyltransferase like 13 (METTL13), which is implicated in protein binding and synthesis, act as oncogenic molecule. This study aimed to reveal the potential mechanism of METTL13 in head and neck squamous cell carcinoma (HNSCC) via mediating enhancing translation efficiency of Snail. Methods: The expression of METTL13, Snail and ki67 were detected in patient HNSCC and paired para-carcinoma tissues. Knockdown and ectopic expression experiments were used in HNSCC cells to investigate their effects on tumor cell migration, proliferation and apoptosis. RNA-sequencing, polysome profiling and SUnSET assays were developed to obtain the further mechanism of the interaction among METTL13, Snail and translation.Results: METTL13 was significantly upregulated in HNSCC at both mRNA and protein level. Increased METTL13 was negatively associated with clinical prognosis. And METTL13 markedly affected HNSCC cellular phenotypes in vivo and vitro. Further mechanism study revealed that METTL13 could regulate EMT signaling pathway by mediating enhancing translation efficiency of Snail, the key transcription factor in EMT, hence regulating the progression of EMT. Conclusion: Altogether, our study had revealed the clinical significance of METTL13 in HNSCC, and providing a potential therapeutic strategy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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