Construction of shuttle vectors for cloning inVibrio cholerae andEscherichia coli

Autor:	Dibyendu K. Panda, Jyotirmoy Das, Rajat Banerjee
Rok vydání:	1995
Předmět:	Plasmid preparation General Medicine Biology Origin of replication medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Transformation (genetics) Plasmid Shuttle vector Vibrio cholerae medicine Replicon General Agricultural and Biological Sciences Escherichia coli
Zdroj:	Journal of Biosciences. 20:367-376
ISSN:	0973-7138 0250-5991
DOI:	10.1007/bf02703840
Popis:	Starting from a naturally occurring cryptic plasmid pVC540 ofVibrio cholerae non-OI. strain 1095, a number of plasmid vectors have been constructed for cloning genes inVibrio cholerae by introducing antibiotic resistance markers containing a set of unique cloning sites. The constructs pVC810 and pVE920 have the origins of bothVibrio cholerae andEscherichia coli replicons and are stable in both organisms in the absence of selective pressure. These plasmids can serve as shuttle vectors betweenEscherichia coli andVibrio cholerae. The plasmid vectors reported here along with the demonstration of transformation inVibrio cholerae by plasmid DNA will facilitate genetic analysis of this important human pathogen.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e64f152f6892c7b1a523b06a9a60499a https://doi.org/10.1007/bf02703840 Zobrazit plný text záznamu Full text from SpringerLink