Measurement of Ceruloplasmin from Its Oxidase Activity in Serum by Use of o-Dianisidine Dihydrochloride

Autor:	H. Peter Lehmann, Myrton F. Beeler, Karl H. Schosinsky
Rok vydání:	1974
Předmět:	Radial immunodiffusion Oxidase test Chromatography medicine.diagnostic_test biology Chemistry Coefficient of variation Biochemistry (medical) Clinical Biochemistry Substrate (chemistry) Biphenyl compound Immunodiffusion Spectrophotometry medicine biology.protein Ceruloplasmin
Zdroj:	Clinical Chemistry. 20:1556-1563
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/20.12.1556
Popis:	We have developed a new method for determination of serum ceruloplasmin from its oxidase activity, in which o-dianisidine dihydrochloride is used as substrate. o-Dianisidine dihydrochloride is more stable than is the more widely used p-phenylenediamine substrate, and forms a stable product that is measured at 540 nm. Correlation was good between results of this method and those obtained with both a p-phenylenediamine substrate method (r = 0.98) and a radial immunodiffusion procedure (r = 0.97). There is little or no interference from reducing or colored components of serum. The coefficient of variation (day-to-day) for the method was 4.2%. A normal range of 62-140 U/ liter has been determined by the reported method.
Databáze:	OpenAIRE
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