Impact of using validated or standard reference genes for miRNA qPCR data normalization in cell type identification

Autor:	M. Sirker, Weibo Liang, Markus A. Rothschild, Iva Gomes, Rolf Fimmers, Lu-Shun Zhang, Peter M. Schneider
Rok vydání:	2015
Předmět:	Genetics Database normalization Normalization (statistics) Mirna expression Reference genes microRNA Mirna profiling Computational biology Biology Gene Pathology and Forensic Medicine
Zdroj:	Forensic Science International: Genetics Supplement Series. 5:e199-e201
ISSN:	1875-1768
DOI:	10.1016/j.fsigss.2015.09.080
Popis:	For the analysis of cell type-specific miRNA expression patterns qPCR is currently the method of choice owing to its high accuracy. However, to obtain reliable results, a proper normalization strategy is an absolute prerequisite, which is often underestimated. To demonstrate the importance of using a set of suitable reference genes, we tested two normalization strategies by comparing gene expressions of tissue-specific miRNA targets normalized against: (1) previously validated endogenous controls (miR92 and miR374) and (2) a commonly used miRNA reference (U6B).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e53e86c367bf28ce30da509f4586aa98 https://doi.org/10.1016/j.fsigss.2015.09.080 Zobrazit plný text záznamu Full Text from ScienceDirect