Chromatographic analysis and purification of multiply tritium-labelled eicosanoids
| Autor: | V. P. Shevchenko, I. Yu. Nagayev, N. F. Myasoyedov |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Nuclear Energy and Engineering Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health lipids (amino acids peptides and proteins) Radiology Nuclear Medicine and imaging Tritium respiratory system Pollution High-performance liquid chromatography Spectroscopy Analytical Chemistry |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry Articles. 121:479-487 |
| ISSN: | 1588-2780 0236-5731 |
| DOI: | 10.1007/bf02041436 |
| Popis: | This is a comparative study of different chromatographic techniques [gas-liquid (GLC), thin-layer (TLC), liquid (LC), high-pressure liquid (HPLC) chromatography] as applied to the analysis and preparative purification of tritium-labelled eicosanoids with a molar radioactivity of 1.8–8.8 TBq/mmol obtained by selective hydrogenation and by chemical or enzymic methods. We demonstrate the possibility of analyzing reaction mixtures and isolating individual multiply labelled eicosanoids with a chemical and radiochemical purity of 95–98%. Special features of HPLC for high molar radioactivity eicosanoids are considered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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