Human skin cell cultures onto PLA50(PDLLA) bioresorbable polymers: Influence of chemical and morphological surface modifications
| Autor: | Jean-Jacques Guilhou, Henri Garreau, Michel Vert, Xavier Garric, Jean-Pierre Molès |
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| Rok vydání: | 2004 |
| Předmět: |
0303 health sciences
Materials science Ethylene oxide Metals and Alloys Biomedical Engineering Human skin 02 engineering and technology 021001 nanoscience & nanotechnology Lactic acid Biomaterials 03 medical and health sciences chemistry.chemical_compound medicine.anatomical_structure chemistry Tissue engineering Cell culture Polymer chemistry Ceramics and Composites medicine Biophysics 0210 nano-technology Keratinocyte Fibroblast Glycolic acid 030304 developmental biology |
| Zdroj: | Journal of Biomedical Materials Research Part A. :180-189 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.30216 |
| Popis: | Poly(alpha-hydroxy acid)s derived from lactic and glycolic acid are bioresorbable polymers which can cover a large range of thermal, physical, mechanical, and biological properties. Human keratinocytes have been shown as able to grow on a poly(DL-lactic acid) film. However the keratinocyte growth was delayed with respect to culture on standard tissue culture polystyrene, even though the same plateau level was observed after 2 weeks. In order to improve the performance of poly(DL-lactic acid) films as skin culture support, their surface was modified by creating tiny cavities using a method based on the leaching out of poly(ethylene oxide) from poly(lactic acid)-poly(ethylene oxide) heterogeneous blends. The surface of the films was also chemically modified by alkaline attack with sodium hydroxide and by type-I collagen coating. Murine fibroblast cell line and primary cultures of human fibroblasts and of two types of keratinocytes were allowed to adhere and to grow comparatively on the different films. The presence of cavities affected neither the adhesion of dermal fibroblasts nor that of keratinocytes. Only keratinocyte proliferation was significantly reduced by the presence of cavities. Collagen coating improved skin cell adhesion and proliferation as well, except in the case of murine fibroblasts. In the case of the NaOH treatments, similar trends were observed but their extent depended on the treatment time. In the case of chemical modifications, fluorescence microscopy bore out adhesion and proliferation tendencies deduced from MTT tests. |
| Databáze: | OpenAIRE |
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