Visualization of the light field of multifocal intraocular lenses using a dual wavelength approach
| Autor: | Kathrin Rubly, Timo Eppig, A. Rawer, Simon Schröder, Achim Langenbucher |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Microscope business.industry General Medicine Multifocal intraocular lens Laser Fluorescence Collimated light law.invention Cuvette 03 medical and health sciences Ophthalmology Wavelength 030104 developmental biology 0302 clinical medicine Optics law 030220 oncology & carcinogenesis Optoelectronics business Light field |
| Zdroj: | Acta Ophthalmologica. 95 |
| ISSN: | 1755-375X |
| Popis: | Purpose To implement a setup which allows simultaneous visualization of the far and near distance light field of multifocal intraocular lenses (MIOL) with two different wavelengths. Methods We used two different laser systems (532 nm and 405 nm) in junction with Powell lenses to create vertical laser lines. One laser line was collimated to simulate far distance imaging while the second laser line was diverging simulating near distance imaging at approximately 0.3 m distance. The MIOL was placed in a glass cuvette filled with balanced saline solution which was doped with two different fluorophores. The fluorophores were chosen to have a large Stokes shift and good separation between their excitation and emission bands. Image acquisition was performed with a slit lamp bio microscope and a digital single reflex camera. We investigated three different IOL types: a bifocal MIOL, a trifocal MIOL and an extended depth of focus IOL. Results We found that Propidium iodide and Fluorescein were appropriate fluorophores. Propidium iodide was excited at 532 nm and showed a red fluorescence while Fluorescein was excited at 405 nm showing the green fluorescence. Both lasers required a high power of approximately 30 mW in order to create a visible fluorescent reaction in the cuvette. All three MIOL types provided a good separation between far and near distance light. The regions of overlapping light fields were visible as yellow fluorescence. Conclusions In contrast to the previously published setups using a single wavelength with collimated excitation light and axially separated far and near distance foci the current setup allows superposition of the far and near distance foci which is more realistic compared to the situation in the eye. The dual wavelength approach enables an easy optical separation of far and near distance light. |
| Databáze: | OpenAIRE |
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