A semi-packed micro GC column for separation of the NAFLD exhaled breath VOCs
| Autor: | Jianhai Sun, Hairong Wang, Baoqing Han, Tinghan Liu, Guishan Wu, Jiuhong Wang, Hao Huang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Alkane Chromatography Materials science 010401 analytical chemistry 02 engineering and technology Surfaces and Interfaces General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Volumetric flow rate Pentane chemistry.chemical_compound chemistry Breath gas analysis Anodic bonding Materials Chemistry Volatile organic compound Gas detector Gas chromatography 0210 nano-technology |
| Zdroj: | Surface and Coatings Technology. 363:322-329 |
| ISSN: | 0257-8972 |
| DOI: | 10.1016/j.surfcoat.2019.02.049 |
| Popis: | The separation and detection of human breath play a significant role in early and non-invasive disease diagnosis. Various volatile organic compound (VOC) gases are considered as the biomarkers of a nonalcoholic fatty liver disease (NAFLD) patient. In this paper, a micro gas chromatography (GC) column used for VOCs separation of the NAFLD breath sample was presented, from its design, fabrication and separation performance testing. The serpentine column made using microelectromechanical system (MEMS) technology was chosen for its relatively small band broadening. The micro pillar configuration was simulated and optimized. The 3 m and 1 m long column with a cross-section of 200 μm × 240 μm was fabricated by deep-reactive-ion-etching (DRIE) method and sealed with anodic bonding. The stationary phase was coated to the inner wall of the column by static coating. The column was installed into the conventional gas chromatography to test separation performance of the GC. The nonpolar alkane mixture containing the NAFLD biomarker gas pentane was separated and the experimental factors such as the temperature and the carrier gas flow rate were studied. The results indicated that the micro GC column can effectively separate alkanes from C5-C12 within 5 min. This could help the micro detector of micro GC system to quickly identify the contents or concentrations of characteristic alkanes. The micro GC column can meet the requirements of the micro GC system for the breath analysis of the NAFLD or other metabolic liver diseases. The micro column assembled with micro gas detector and preconcentrator can be hopefully developed into a portable breath analysis device. |
| Databáze: | OpenAIRE |
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