Interactions of plasma kallikrein and C1-s with normal and dysfunctional C1(-)-inhibitor proteins from patients with hereditary angioneurotic edema: analytic gel studies
| Autor: | Betty Tsuei, Virginia H. Donaldson, Richard A. Harrison, George Kindness, David H. Bing, Constance J. Wagner, Fred S. Rosen |
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| Rok vydání: | 1987 |
| Předmět: |
Gel electrophoresis
medicine.medical_specialty biology Hereditary angioneurotic edema Immunology Cell Biology Hematology Kallikrein bacterial infections and mycoses Cleavage (embryo) Biochemistry Molecular biology respiratory tract diseases C1-inhibitor chemistry.chemical_compound Endocrinology chemistry Internal medicine Mole Blood plasma medicine biology.protein heterocyclic compounds Sodium dodecyl sulfate |
| Zdroj: | Blood. 69:1096-1101 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v69.4.1096.1096 |
| Popis: | Purified preparations of normal C1(-)-inhibitor (C1(-)-INH) formed high mol wt complexes with plasma kallikrein that were stable during sodium dodecyl sulfate (SDS)-gel electrophoresis, but most of the dysfunctional C1(-)-INH proteins isolated from plasma of patients with type II hereditary angioneurotic edema (HANE) did not. Two of eight dysfunctional C1(-)-INH proteins were cleaved to lower mol wt forms that were not seen following the reaction of normal C1(-)-INH with equimolar amounts, or less, of plasma kallikrein. Only the higher mol wt component of normal C1(-)-INH (106,000 mol wt) appeared to form a stable complex with the plasma kallikrein, whereas both the 106,000 and 96,000 mol wt forms made stable complexes with C1-s. When a preparation of normal C1(-)-INH containing a homogeneous single band of C1(-)-INH was exposed to C1-s or kallikrein, a “doublet” form evolved in which the heaviest band was in the original position of native C1(-)-INH; C1- s cleavage provided a second band of 96,000; and cleavage by kallikrein, a second band of 94,000 mol wt. We conclude that dysfunctional C1(-)-INH proteins from plasma of persons with type II hereditary angioneurotic edema have impaired interactions with plasma kallikrein and are heterogeneous with respect to these interactions. Moreover, the requirements for the formation of stable complexes between normal C1(-)-INH and plasma kallikrein differed from those for stable complex formation with C1-s. The doublet form of C1(-)-INH, which purified preparations frequently demonstrate, may be due to prior cleavage by C1-s or kallikrein. |
| Databáze: | OpenAIRE |
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