| Autor: |
Gertine W. van Oord, Jasmin Joseph-Chazan, Raymond T. Chung, Jordan J. Feld, Juan D. Sanchez Vasquez, Chris Oetheimer, Martin Arreola Villanueva, Ira Fleming, Nir Hacohen, Alex S. Genshaft, Georg M. Lauer, Nancy Tran, Sonu Subudhi, Alex K. Shalek, Jacques Bollekens, Lauke L. Boeijen, Nadia Conceição-Neto, Aman Mehrotra, Adam J. Gehring, Nadia Alatrakchi, Arlin Keo, Constantine Tzouanas, Boris J. B. Beudeker, Zgjim Osmani, Robert J. de Knegt, Mike Vilme, Jeroen Aerssens, Harmen J.G. van de Werken, Harry L.A. Janssen, Shirin Nkongolo, Deeqa Mahamed, Andre Boonstra, Zwier M. A. Groothuismink, Riley Drake |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.1101/2021.11.30.470634 |
| Popis: |
Blood samples are frequently collected in human studies of the immune system but poorly represent tissue-resident immunity. Understanding the immunopathogenesis of tissue-restricted diseases, such as chronic hepatitis B, necessitates direct investigation of local immune responses. We developed a workflow that enables frequent, minimally invasive collection of liver fine-needle aspirates in multi-site international studies and centralized single-cell RNA sequencing data generation using the Seq-Well S3 picowell-based technology. All immunological cell types were captured, including liver macrophages, and showed distinct compartmentalization and transcriptional profiles, providing a systematic assessment of the capabilities and limitations of peripheral blood samples when investigating tissue-restricted diseases. The ability to electively sample the liver of chronic viral hepatitis patients and generate high-resolution data will enable multi-site clinical studies to power fundamental and therapeutic discovery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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