Capillary electrophoresis-mass spectrometry
Autor: | Jianyi Cai, Jack D. Henion |
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Rok vydání: | 1995 |
Předmět: |
Chromatography
Chemistry Electrospray ionization Organic Chemistry Analytical chemistry General Medicine Fast atom bombardment Mass spectrometry Biochemistry Capillary electrophoresis–mass spectrometry Fourier transform ion cyclotron resonance Analytical Chemistry Capillary electrophoresis Isotachophoresis Ion trap |
Zdroj: | Journal of Chromatography A. 703:667-692 |
ISSN: | 0021-9673 |
Popis: | As an on-line separation method, capillary electrophoresis (CE)-mass spectrometry (MS) distinguishes analytes by both their differences in electrophoretic mobilities and structural information. CE-MS combines the advantages of CE and MS so that information on both high separation efficiency and molecular masses and/or fragmentation can be obtained in one analysis. During the past few years CE-MS has undergone significant development both in instrumentation and application. Several ionization methods have been used for CE-MS. These include electrospray ionization, ion spray or pneumatically assisted electrospray ionization, and continuous-flow fast atom bombardment. The direct coupling of CE to desorption MS has not yet been reported, although publications have appeared on the off-line coupling of CE with matrix-assisted laser desorption ionization and 252Cf plasma desorption MS using fraction collection. Numerous new applications of CE-MS have been published in the areas of biological sciences, pharmaceutical and drug metabolism, and environmental analysis. The majority of applications of CE-MS have been in the field of biological and biochemical studies. Several limitations associated with CE-MS have precluded the technique being widely accepted for routine analysis. The major limitation is its relatively poor concentration sensitivity. The concentration detection limits of currently available CE-MS instrumentation are too high for most real-world applications. Other drawbacks with CE-MS include the fluctuation in analyte migration time and limitations in electrolyte selections. Approaches to improve the concentration sensitivity of CE-MS include on-line preconcentration either by capillary isotachophoresis or chromatographic methods. Another solution to increasing the sensitivity of CE-MS is the development of alternative types of mass spectrometers which offer the potential for greater sensitivity, such as ion trap, Fourier transform ion cyclotron resonance, and time-of-flight (TOF) mass spectrometers. Coated capillaries are useful in improving separation efficiencies of biomolecules by minimizing their adsorption onto the CE capillary walls. At present, CE-MS is still not generally considered for routine analysis mainly due to its limited concentration sensitivity. As a complementary separation method to LC-MS with extremely high efficiency, CE-MS has the potential for wide acceptance in the future. The popularity of CE-MS will continue to grow, as more sensitive MS instrumentation and CE-MS interface are developed. |
Databáze: | OpenAIRE |
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