| Autor: |
Mykhailo Huniadi, Simon Salanci, Jan Sykora, Slavomir Kurhajec, Marek Sarissky |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
European Pharmaceutical Journal. 69:87-89 |
| ISSN: |
2453-6725 |
| Popis: |
SAP and EAT-2 are intracellular adaptor proteins that link SLAMF receptors to downstream intracellular signalling pathways. Their expression and functions vary among different types of hematopoietic cells. The roles of SAP and EAT-2 in haematological malignancies are poorly understood. Here, we attempted to optimise a method for the detection of intracellular SAP and EAT-2 by flow cytometry. We found that using PerFix EXPOSE kit for cell fixation and permeabilization ensures optimal results in terms of staining intensity and signal-to-noise ratio. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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