Detection of Prethrombin 1 in Human Blood Plasma
| Autor: | Platonova Tn, E. V. Lugovskoy, V. O. Chernyshenko, D S Korolova, Chernyshenko Tm |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology Human blood business.industry General Medicine medicine.disease Gastroenterology Abdominal aortic aneurysm 03 medical and health sciences 030104 developmental biology Thrombin In vivo Internal medicine Blood plasma Medicine In patient business Stroke Coagulation Disorder circulatory and respiratory physiology medicine.drug |
| Zdroj: | International Blood Research & Reviews. 5:1-7 |
| ISSN: | 2321-7219 |
| DOI: | 10.9734/ibrr/2016/24683 |
| Popis: | Background: Prethrombin 1 is one of prothrombin derivatives which appears in the presence of thrombin in vitro. However, existence of prethrombin 1 in vivo remained questionable. The aim of present work was to detect of prethrombin 1 in vivo at abdominal aortic aneurysm, hip replacement after fracture and stroke. Methodology: Blood plasma samples of patients with abdominal aortic aneurysm, hip replacement after fracture, stroke, and patients which were treated with warfarine were collected. Detection of prethrombin 1 was completed using three independent approaches: combination of prothrombin index and ecamulin (ecarin) index; the APTT-test modified by addition of exogenous prothrombin; Western-blotting of blood plasma using polyclonal antibody to prothrombin. Results: Prethrombin 1 presence in patients’ blood plasma at studied pathologies was proven. Inactive prothrombin (descarboxy-prothrombin), but not prethrombin 1 was found in blood plasma of patients which were treated with warfarine. Developed approach can be used for testing the prethrombin 1 in clinical diagnostics. Conclusion: Prethrombin 1 at different pathologies, accompanied by coagulation disorders was detected in patients’ blood plasma directly. |
| Databáze: | OpenAIRE |
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