Direct analysis of free-base nicotine in tobacco leaf by headspace solid-phase micro-extraction combined with gas chromatography/mass spectrometry

Autor: Tougen Liao, Liang He, Haowei Yang, Wang Meng, Li Tang, Baokun Zhu
Rok vydání: 2019
Předmět:
Zdroj: Accreditation and Quality Assurance. 24:341-349
ISSN: 1432-0517
0949-1775
DOI: 10.1007/s00769-019-01381-z
Popis: A little nicotine in tobacco leaf is non-ionized, which is also known as free-base nicotine (FBN). The dynamic equation existed between FBN and ionized nicotine can easily change with water content, temperature and pH value. In this study, a method of the direct quantitative analysis for FBN in tobacco leaf by using headspace solid-phase micro-extraction, combined with gas chromatography/mass spectrometry, was developed. The optimum conditions for analysis method, including sample forms, SPME fiber type, pre-equilibrium temperature, pre-equilibrium time, extraction temperature and extraction time, were investigated in detail. Under the optimized conditions, the linear range of the method was 1 mg/mL–100 mg/mL, with a correlation coefficient (r) of 0.9977 and a detection limit of 0.84 μg/g, the repeatability (expressed as relative standard deviation) was 3.35 %, and the recovery of this method was between 99.56 % and 99.80 %. The uncertainty budget was finally done according to the “Guide to the Expression of Uncertainty in Measurement,” and the relative expanded uncertainty was 7.1 % with coverage factor k = 2. This method may be reliable and convenient for tobacco control.
Databáze: OpenAIRE
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