| Autor: |
Paul R. Haddad, G. K. C. Low, A.M. Duffield |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 336:15-24 |
| ISSN: |
0378-4347 |
| DOI: |
10.1016/s0378-4347(00)85126-9 |
| Popis: |
A simple retention model is advanced to explain the observation that a chromatographically pure solute can produce up to three discrete peaks under suitable mobile-phase conditions in reversed-phase, ion-pair high-performance liquid chromatography. This model is based on the proposal that peak splitting results from the interplay of two distinct retention mechanisms such as ion pairing or dynamic ion exchange. Transition between these two mechanisms in induced under certain mobile-phase conditions, for example the addition of suitable quantities of sodium sulphate to the eluent. The suggested model is used for the computer calculation of eluted peak profiles and these were found to closely resemble experimentally observed peak shapes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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