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AimsThe gut microbiota is referred to an ‘extra organ’ and is ciritical in assisting the host in terms of nutrition and immunity. Environmental stressors could alter gut microbial community and cause gut inflammation. This study aimed to investigate and compare the gut microbiota community between healthy and diseasedTor tambroides.Methodology and resultsIn this study, such gut microbial alterations were explored using NGS-based 16S rDNA sequencing on the Malaysian mahseer (T. tambroides). Three adult healthy and three diseased adult Malaysian mahseers (showing signs of exophthalmia, coelomic distension and petechial haemorrhage) were obtained from LTT Aquaculture Sdn Bhd. Our results revealed significant differences in microbial diversity, composition and function between both populations ofT. tambroides.Alpha diversity analysis depicts lower diversity of gut microbiota composition in diseasedT. tambroidesas compared to the healthy group. In particular,Enterobacteriaceae,Aeromonas, Bacteroides, VibrioandPseudomonaswere found within gut microbiota of the diseased fishes. In addition, cellulose-degrading bacteria and protease-producing bacteria were identified from the gut ofT. tambroides.Conclusion significance and impact of studyThus, our findings emphasised on the association between the alteration in gut microbiota composition and infectious abdominal dropsy (IAD) inT. tambroides.This finding is important to provide basic information for further diagnosis, prevention and treatment of intestinal diseases in fish. |
