Control of pH by CO2 Pressurization for Enzymatic Saccharification of Ca(OH)2-Pretreated Rice Straw in the Presence of CaCO3

Autor:	Ken Tokuyasu, Masakazu Ike
Rok vydání:	2020
Předmět:	biology Chemistry Aspergillus niger food and beverages chemistry.chemical_element Cellulase Raw material Calcium biology.organism_classification Hydrolysis Enzymatic hydrolysis biology.protein Sugar Trichoderma reesei Nuclear chemistry
Zdroj:	Journal of Applied Glycoscience. 67:59-62
ISSN:	1880-7291 1344-7882
DOI:	10.5458/jag.jag.jag-2019_0019
Popis:	The aim of this study was to investigate the effect of pH control by CO 2 pressurization on the enzymatic hydrolysis of herbaceous feedstock in the calcium capturing by carbonation (CaCCO) process for fermentable sugar production. The pH of the slurry of 5 % (w/w) Ca(OH) 2 -pretreated/CO 2 -neutralized rice straw could be controlled between 5.70 and 6.38 at 50 °C by changing the CO 2 partial pressure ( p CO 2 ) from 0.1 to 1.0 MPa. A mixture of fungal enzyme preparations, namely, Trichoderma reesei cellulases/hemicellulases and Aspergillus niger β-glucosidase, indicated that pH 5.5-6.0 is optimal for solubilizing sugars from Ca(OH) 2 -pretreated rice straw. Enzymatic saccharification of pretreated rice straw under various p CO 2 conditions revealed that the highest soluble sugar yields were obtained at p CO 2 0.4 MPa and over, which is consistent with the expected pH at the p CO 2 without enzymes and demonstrates the effectiveness of pH control by CO 2 pressurization.
Databáze:	OpenAIRE
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