Utilization of an osmium complex as a sequence recognizing material for DNA-immobilized electrochemical sensor

Autor:	Yuji Mishima, Sanae Ikeda, Junko Motonaka
Rok vydání:	1997
Předmět:	inorganic chemicals Tris endocrine system Chemistry Inorganic chemistry chemistry.chemical_element Sequence (biology) Electrochemistry Biochemistry Analytical Chemistry Electrochemical gas sensor chemistry.chemical_compound Electrode Environmental Chemistry A-DNA Osmium Spectroscopy DNA
Zdroj:	Analytica Chimica Acta. 345:45-50
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(97)00045-7
Popis:	A novel immobilized DNA electrode based on a porous carbon electrode was prepared to study interactions between DNA and chemical compounds. The characteristics of this electrode were evaluated by electrochemical methods, and then the interaction between DNA and an osmium complex was studied. On this electrode (electrode surface; 0.66–2.37 cm2), DNA was immobilized at 0.26 × 10−4 − 1.23 × 10−4 mol cm−2. Hybridization of DNA was observed with a relative standard deviation of 11% (n = 4). The tris(bipyridyl)osmium(II/III) ion (osmium complex) was found to interact to major or minor grooves. Using this osmium complex, a DNA sequence could be recognized with more than 12 mer of the same sequence.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dd108c5b96b3a7dcf56700984ac31f9a https://doi.org/10.1016/s0003-2670(97)00045-7 Zobrazit plný text záznamu Full Text from ScienceDirect