Unraveling the Causes of Failed Fertilization After Intracytoplasmic Sperm Injection Due to Oocyte Activation Deficiency
| Autor: | Björn Heindryckx, Ramesh Reddy Guggilla, Petra De Sutter, Davina Bonte, Panagiotis Stamatiadis |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Infertility 030219 obstetrics & reproductive medicine urogenital system medicine.medical_treatment Oocyte activation Failed fertilization Biology Diagnostic tools medicine.disease Intracytoplasmic sperm injection World health Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Human fertilization medicine reproductive and urinary physiology |
| DOI: | 10.1016/b978-0-12-812571-7.00015-0 |
| Popis: | Infertility affects people worldwide and has been classified as a disease by the World Health Organization (WHO). Although intracytoplasmic sperm injection (ICSI) is a powerful technique, complete fertilization failure still occurs in 1%–5% of ICSI cycles. This failure is mainly attributed to an oocyte activation deficiency that can be sperm-related or oocyte-related. This chapter summarizes the state of the art of the oocyte activation mechanism, stressing the importance of calcium transients in this process. Furthermore, we describe the different sperm-related and oocyte-related factors that are involved in this oocyte activation process, which could be linked to fertilization failure, with special focus on the sperm-activating factor phospholipase C zeta (PLCζ). We present the different diagnostic tools currently available to reveal the oocyte-activating capacity of human spermatozoa. Finally, the clinical applications to overcome fertilization failure after ICSI are described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|