Regulation of Granulosa Cell-Derived Insulin-Like Growth Factor Binding Proteins (IGFBPs): Role for Protein Kinase-C in the Pre- and Posttranslational Modulation of IGFBP-4 and IGFBP-51
| Autor: | Eli Y. Adashi, Doo Seok Choi, Diran Chamoun, Eliahu Levitas, Laurence C. Udoff, Ron G. Rosenfeld, Adriano B. Tavares, Carol E. Resnick |
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| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
biology Activator (genetics) Granulosa cell Growth factor medicine.medical_treatment Cell Biology General Medicine Insulin-like growth factor-binding protein chemistry.chemical_compound Endocrinology Reproductive Medicine chemistry Internal medicine medicine biology.protein Phorbol Staurosporine Signal transduction Protein kinase C medicine.drug |
| Zdroj: | Biology of Reproduction. 67:1003-1012 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.101.001214 |
| Popis: | A growing body of information suggests antigonadotropic and atretogenic roles for granulosa cell-derived insulin-like growth factor binding proteins (IGFBPs) 4 and 5 during ovarian folliculogenesis. Activation of protein kinase-A (PKA) in rat granulosa cells has been shown to modulate the relative expression of IGFBP-4 and -5 transcripts and proteins. In this article, we assess the role of protein kinase-C (PKC) in this regard. Provision of granulosa cells with phorbol 12-myristate 13-acetate (PMA) (but not 4αPMA, an inert analogue), a tumor-promoting phorbol ester and an established activator of PKC, was without significant effect on the expression of IGFBP-4 transcripts but resulted in biphasic dose-dependent alterations in IGFBP-5 transcripts and in the accumulation of the IGFBP-4 and -5 proteins. Comparable effects were noted for GnRH, an established PKC agonist. Provision of staurosporine, a potent inhibitor of the catalytic subunit of PKC, produced significant dose-dependent decrements in t... |
| Databáze: | OpenAIRE |
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