Adsorption of Cellulase Isolated fromAspergillus Nigeron Chitosan/Alginate Particles Functionalized with Epichlorohydrin

Autor:	Wellington S. Adriano, Bruna Tarciana Cavalcante Bezerra, Ivanildo J. Silva, Elaine C. Rodrigues, Rodrigo Silveira Vieira, Diana C. S. Azevedo, Barbara V. Farias
Rok vydání:	2013
Předmět:	Chromatography biology Elution General Chemical Engineering Aspergillus niger Surfaces and Interfaces General Chemistry Cellulase biology.organism_classification Chitosan chemistry.chemical_compound Adsorption chemistry Sodium citrate biology.protein Epichlorohydrin Particle size
Zdroj:	Adsorption Science & Technology. 31:17-34
ISSN:	2048-4038 0263-6174
Popis:	This work investigates the adsorption of cellulase (isolated from Aspergillus niger) in a fixed-bed column using chitosan/alginate particles functionalized with epichlorohydrin (Cs/Ag-E) as the adsorbent. The particles were prepared and characterized with respect to apparent density, particle porosity, particle size, surface chemistry and thermal stability. Cellulase solutions were prepared in sodium citrate buffer (20 mM) at pH 5. Several breakthrough curves were obtained by varying the flow rate and feed concentrations in order to evaluate the suitable bed height and determine the adsorption isotherm, respectively. The adsorption isotherm fitted well with Langmuir equation with maximum adsorption capacity of about 11 mg of cellulase/g of adsorbent. Successive cycles (adsorption/washing/elution) were performed and the results demonstrate the reversibility of the process and its potential for enzyme purification and separation. However, cellulase activity in the eluted fraction is only 29% of that of the fresh enzyme fed to the column.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dc6512ac7712d6a491e9c0dc87d25af0 https://doi.org/10.1260/0263-6174.31.1.17 Zobrazit plný text záznamu Plný text ve formátu PDF