Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures

Autor:	Philippe Marmey, N'Da Koffi Konan, Roger N. Beachy, R. Carcamo, Graham G. Henshaw, Claude M. Fauquet, C. Schöpke, N. J. Taylor
Rok vydání:	1996
Předmět:	Reporter gene Somatic embryogenesis Transgene fungi Biomedical Engineering food and beverages Bioengineering Genetically modified crops Biology Applied Microbiology and Biotechnology Molecular biology Genetically modified organism Tissue culture Callus Botany Molecular Medicine Gene Biotechnology
Zdroj:	Nature Biotechnology. 14:731-735
ISSN:	1546-1696 1087-0156
DOI:	10.1038/nbt0696-731
Popis:	A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene encoding neomycin phosphotransferase (nptII) and a gene encoding beta-glucuronidase (uidA). Selection of bombarded tissue with paromomycin resulted in the establishment of putative transgenic embryogenic calli. In most of these calli, beta-glucuronidase was detected histochemically. Molecular analysis of paromomycin-resistant embryogenic calli and of plants regenerated from these calli, confirmed the stable integration of bombarded DNA into the cassava genome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::db307fdb1c4c5deb6011eb94d013a42f https://doi.org/10.1038/nbt0696-731 Zobrazit plný text záznamu