Prevalence and characterization of Esherichia coli isolated from apparently healthy and diseased Cockatiles and budgerigars

Autor: A. A. R. Khafagy, M. A. Moustafa, I. S. Ibrahim, Shabana, I.I., I. M. F. Khalifa
Rok vydání: 2015
Předmět:
DOI: 10.5281/zenodo.27223
Popis: A total of 258 fecal samples from Cockatiles and budgerigars (230 from apparently healthy birds and 28 from clinically diseased) were collected. The bacteriological examination revealed the isolation of E.coli (51.28%), Salmonella (10.26%), Klebsiella (17.95%), Proteus (17.95%) and Enterobacter (2.56%) respectively in relation to total number of isolates. They were infected with percentage of E.coli (7.75%), Salmonella (1.55%), Klebsiella (2.71%), proteus (2.71%) and Enterobacter (0.39%) respectively in relation to total number of collected samples. The isolated E.coli serovars were belonging to O1 (13.3%), O2 (20%), O26 (6.7%) and O untypable (60%). E.coli strains were then examined for enterotoxin production, E.coli O26 and Ountypable was heat labile toxin producer [LT]. While O1, O2, Ountypable were verotoxin producer. On the other hand, no heat stable toxin producer strains [ST] were detected. RAPD PCR profile was used for differentiation between E.coli different serotypes and revealed a significant difference among the revealed serotypes. The antibiotic sensitivity tests revealed that, ciprofloxacin and gentamycin were the most effective drugs against the isolated E.coli.
Databáze: OpenAIRE