PMC-12, a Prescription of Traditional Korean Medicine, Improves Amyloidβ-Induced Cognitive Deficits through Modulation of Neuroinflammation

Autor: Jung Hwa Park, Young-Whan Choi, Jin Ung Baek, Jaewon Lee, Yeon Suk Jung, Hwa Kyoung Shin, Byung Tae Choi, Min Young Park, Cheol Min Kim
Rok vydání: 2015
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine. 2015:1-10
ISSN: 1741-4288
1741-427X
DOI: 10.1155/2015/768049
Popis: PMC-12 is a prescription used in traditional Korean medicine that consists of a mixture of four herbal medicines,Polygonum multiflorum,Rehmannia glutinosa,Polygala tenuifolia, andAcorus gramineus, which have been reported to have various pharmacological effects on age-related neurological diseases. In the present study, we investigated whether PMC-12 improves cognitive deficits associated with decreased neuroinflammation in an amyloid-β-(Aβ-) induced mouse model and exerts the antineuroinflammatory effects in lipopolysaccharide-(LPS-) stimulated murine BV2 microglia. Intracerebroventricular injection ofAβ25-35in mice resulted in impairment in learning and spatial memory, whereas this was reversed by oral administration of PMC-12 (100 and 500 mg/kg/day) in dose-dependent manners. Moreover, PMC-12 reduced the increase of Aβexpression and activation of microglia and astrocytes in theAβ25-35-injected brain. Furthermore, quantitative PCR data showed that inflammatory mediators were significantly decreased by administration of PMC-12 in Aβ-injected brains. Consistent with thein vivodata, PMC-12 significantly reduced the inflammatory mediators in LPS-stimulated BV2 cells without cell toxicity. Moreover, PMC-12 exhibited anti-inflammatory properties via downregulation of ERK, JNK, and p38 MAPK pathways. These findings suggest that the protective effects of PMC-12 may be mediated by its antineuroinflammatory activities, resulting in the attenuation of memory impairment; accordingly, PMC-12 may be useful in the prevention and treatment of AD.
Databáze: OpenAIRE
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