The Effect of Resveratrol on Bone Status in Rats with Bile Duct Obstruction

Autor:	Pavel Zivny, Stanislav Micuda, Helena Zivna, Eva Brcakova-Dolezelova
Rok vydání:	2013
Předmět:	Liver injury Bone mineral medicine.medical_specialty biology business.industry Bile duct Resveratrol medicine.disease Bone tissue Bone remodeling chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Osteocalcin biology.protein Medicine Femur business
Zdroj:	Open Journal of Endocrine and Metabolic Diseases. :46-51
ISSN:	2165-7432 2165-7424
DOI:	10.4236/ojemd.2013.31007
Popis:	Objective: We studied the influence of bile duct obstruction (BDO) after 28 days on bone metabolism status and their modification by resveratrol in male Wistar rats. Methods: The rats were divided into 3 groups: Sham group with laparotomy and vehiculum application, group BDO—bile duct obstruction and vehiculum application, and group RBDO—bile duct obstruction and resveratrol application (10 mg/kg dose of resveratrol orally once daily). The bone mineral density (BMD; g/cm2) and body composition were measured by dual energy X-ray absorptiometry. The physiccal strenght of femur was examined by controlled break biomechanical testing. The osteocalcin, procollagen type I N-terminal propeptide (PINP) and carboxy-terminal collagen crosslinks (CTX) were analysed by EIA in the bone tissue homogenate. Results: The total BMD was lower in group BDO (0.176 ± 0.005; p = 0.01) and R-BDO (0.181 ± 0.004; p = 0.052) vs Sham (0.209 ± 0.003). BMD of femur diaphysis in BDO group decreased significantly (p = 0.01) but not in R-BDO animals (p = 0.052). Body fat (g, median) was lower in BDO (19.0) and R-BDO (26.0) than in Sham (35.0). The force needed for fracture of femurs (N) significantly decreased in BDO (154 ± 6) and R-BDO (151 ± 13) vs Sham group (208 ± 7). The force needed for neck fracture decreased in BDO (105 ± 3) and R-BDO (115 ± 8) vs Sham (135 ± 9). The osteocalcin decrease in group R-BDO (2.9 ± 0.1) vs Sham (3.7 ± 0.4). Higher PINP/CTX were in BDO (20 ± 5/0.49 ± 0.08) and R-BDO (30 ± 4/0.55 ± 0.07) vs Sham (16 ± 4/0.48 ± 0.11). Conclusions: Our findings suggest that subchronic bile duct obstruction decreased body fat quantity and BMD with decrease of bone rigidity. Resveratrol showed protective effect on liver injury and consequently positive influence on bone tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::d991a75db550f7c01ec2be928bd066ac https://doi.org/10.4236/ojemd.2013.31007 Zobrazit plný text záznamu