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Purpose: Breastfeeding has been endorsed by the World Health Organization as the most effective method to feed infants. To find out the breastfeeding practices and examine the factors affecting the practice among mothers attending immunization clinics at Primary Health Centers within Ado-Ekiti in Nigeria. Methodology: A total of 450 respondents were randomly selected with Fisher’s formula using a cross-sectional descriptive study design. Multistage sampling techniques was used to select two out of fifteen wards in Ado Local Government Area through random sampling by balloting, out of which two Primary Health Care was selected one from each Local Government Area. Adapted, semi-structured, interview administered questionnaire was used, data was entered and analyzed with Statistical Package for Social Sciences version 25 and p value set at 5%. Findings: Out of the respondents, 152 (33.8%) exclusively breastfed their babies and 163 (36.2%) initiated breastfeeding within one hour after delivery. One hundred and thirty-two (29.3%) gave pre-lacteal feeding to their babies, of whom 93 (70.4%) gave water and glucose. From the multivariate analysis, the mothers that were employed were more likely to exclusively breastfed their babies with OR of 1.58 and p value of |